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| Product Name | Mouse Pan B Cell Isolation Kit |
| Catalog No. | ATR-SPS-0039 |
| Description | A column-free magnetic kit for enriching broad mouse B-cell populations, including B1 cells, B2 cells and plasma cells. Non-target cells are labeled and magnetically removed, leaving the desired B-cell fraction unlabeled. |
| Intended Use | Isolation of pan-B-cell populations from mouse spleen and lymph-node single-cell suspensions. |
| Principle / Technology | Negative selection using biotinylated antibodies and streptavidin magnetic beads. |
| Detection Method | Magnetic cell separation followed by flow-cytometric purity assessment. |
| Sample Type | Mouse spleen, lymph node and mixed lymphoid-tissue single-cell suspensions. |
| Performance Range / Specifications | Typical separation time: approximately 16 minutes; reported purity: 96-99%; package capacity: up to 1 × 10^9 cells. |
| Reaction Conditions / Protocol | Incubate the cell suspension with the negative-selection antibody cocktail, add streptavidin magnetic beads and remove labeled non-B cells using a magnetic separator. |
| Components / Formulation | Biotinylated cell-isolation antibody cocktail and streptavidin magnetic beads. |
| Storage Conditions | Store at 2-8°C. Do not freeze. |
| Package Specifications | For up to 1 × 10^9 cells. |
| Product Form | Column-free magnetic cell-isolation kit. |
| Key Features | Broad B-cell population recovery; 96-99% reported purity; approximately sixteen-minute workflow; bead-free target cells; no abnormal activation; no separation column. |
| Shipping Conditions | Ship with ice packs. |
| Compatibility | Compatible magnetic separators, flow cytometry, cell culture and functional assays. |
| Application Notes / Precautions | Use a well-dispersed single-cell suspension and prevent magnetic-bead settling by mixing components thoroughly before use. |
For research use only, not for clinical use.
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