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| Product Name | Phospholipids Colorimetric Assay Kit |
| Catalog No. | BLTR-0008 |
| Description | This phospholipids colorimetric assay kit uses a series of enzymatic reactions to quantify phospholipids in various biological samples. It has high sensitivity, a wide linear range, and low interference, which is beneficial for studies on cell membrane - related lipid metabolism and signal transduction pathways |
| Test Target | Phospholipids |
| Methodology | Enzymatic colorimetric (phospholipase D, choline oxidase, peroxidase) |
| Measurement Range | 0.1-5 mmol/L |
| Sample Type | Serum, plasma, cell membranes, tissue homogenates |
| Assay Time | 15 min at 37°C |
| Wavelength | 500 nm |
| Linearity | 0.1-5 mmol/L |
| Precision (Intra-assay CV) | ≤2.5% |
| Precision (Inter-assay CV) | ≤3.5% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 10 days at 2-8°C |
| Interference (Hemoglobin) | ≤400 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤15 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤800 mg/dL (no significant interference) |
| Reagent Composition | Phospholipase D, choline oxidase, peroxidase, 4 - aminoantipyrine, phenol |
| Reagent Form | Liquid |
| Reagent Volume | R1: 50 mL; R2: 10 mL |
| Calibration Type | External calibration |
| Calibration Levels | 3 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Quantitative determination of phospholipids in biological samples for research on cell membrane structure, lipid transport, and signal transduction |
| Cross - Reactivity | No significant cross - reactivity with triglycerides, cholesterol |
| pH Range | 6.8-7.2 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C |
For research use only, not for clinical use.
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