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| Product Name | phi29 DNA Polymerase, High Concentration (100 U/uL) |
| Catalog No. | IVDRM-0573 |
| Description | Derived from a Bacillus subtilis phage and engineered for outstanding strand displacement and processive synthesis capability, this phi29 DNA Polymerase synthesises DNA fragments up to 70 kb. Its strong 3 prime to 5 prime exonuclease proofreading activity gives high fidelity synthesis, and because of that activity primers with modified 3 prime ends are recommended to prevent degradation. Applications include whole genome amplification, rolling circle amplification and multiple displacement amplification, as well as in vitro synthesis of plasmids, and one unit incorporates 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C |
| Intended Use | Whole genome amplification, rolling circle amplification, multiple displacement amplification and plasmid synthesis |
| Principle / Technology | Highly processive strand-displacing synthesis with strong 3 prime to 5 prime proofreading |
| Sample Type | Circular templates and genomic DNA for amplification |
| Specificity | Synthesis up to 70 kb; primer 3 prime end modification recommended to limit proofreading degradation |
| Reaction Conditions / Protocol | Combine with primers and dNTPs and incubate at 30°C for isothermal amplification |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 1 mL; 5 mL; 25 mL |
| Key Features | High concentration format for large reaction volumes; strong processivity and proofreading fidelity |
| Concentration | 100 U/uL |
| Shipping Conditions | Dry ice shipping |
| Readouts / Downstream Assays | Yield and length profile of amplified DNA |
For in vitro diagnostic use.
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