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| Product Name | phi29 DNA Polymerase for MDA and Rolling Circle Amplification (10 U/uL) |
| Catalog No. | IVDRM-0422 |
| Description | Originally from a Bacillus subtilis phage and reworked through genetic engineering, phi29 DNA Polymerase combines exceptional strand displacement with high processivity and can synthesise DNA fragments up to 70 kb. Its 3 prime to 5 prime proofreading exonuclease activity gives fidelity above nearly all other DNA polymerases, and pg level template inputs are amplified effectively, with 100 ng of template yielding up to 2 ug/uL product. Primers with 3 prime terminal modifications are recommended to guard against proofreading degradation. Typical applications span multiple displacement amplification, rolling circle amplification, whole genome amplification and SNP genotyping. |
| Intended Use | Multiple displacement amplification, rolling circle amplification, whole genome amplification and SNP detection |
| Principle / Technology | Strand displacement isothermal DNA synthesis with 3 prime to 5 prime proofreading exonuclease activity |
| Performance Range / Specifications | Amplifies products up to 70 kb; effective on pg level DNA templates; 100 ng input yields up to 2 ug/uL |
| Specificity | Fidelity higher than most DNA polymerases; 3 prime modified primers recommended to prevent degradation |
| Reaction Conditions / Protocol | Used for MDA, RCA and WGA reactions at 30°C in isothermal workflows |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U; 1000 U; 5000 U |
| Quality Control | No nickase residue contamination detected |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Amplified DNA yield and length by gel or fluorometric quantification |
For in vitro diagnostic use.
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