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| Product Name | Penicillin-Streptomycin Solution, 100x, 10,000 U/mL |
| Catalog No. | ATR-CCR-0013 |
| Description | A sterile 100-fold concentrated antibiotic solution containing 10,000 units/mL penicillin G and 10,000 µg/mL streptomycin sulfate in 0.85% saline, for the prevention of bacterial contamination in mammalian cell culture. |
| Intended Use | Routine antibiotic supplementation of cell culture media to prevent gram-positive and gram-negative bacterial contamination in research cell culture laboratories. |
| Principle / Technology | Penicillin inhibits bacterial cell wall synthesis by binding to penicillin-binding proteins and blocking transpeptidation; streptomycin binds to the 30S ribosomal subunit causing misreading of mRNA and inhibition of protein synthesis. |
| Detection Method | Antimicrobial efficacy testing with challenge organisms, cell cytotoxicity assessment |
| Sample Type | Mammalian cell culture media of all types |
| Performance Range / Specifications | Working concentration 100 U/mL penicillin and 100 µg/mL streptomycin at 1:100 dilution; effective against most gram-positive and gram-negative bacteria |
| Sensitivity / LOD | N/A |
| Specificity | Broad spectrum: gram-positive cocci, gram-negative rods; ineffective against mycoplasma, fungi, and most viruses |
| Reaction Conditions / Protocol | Add 5 mL per 500 mL medium (1:100 dilution); maintain at the working concentration for the duration of culture. |
| Components / Formulation | Penicillin G sodium 10,000 U/mL, Streptomycin sulfate 10,000 µg/mL in 0.85% saline, sterile-filtered |
| Storage Conditions | -20°C (long term); 2–8°C after thawing (use within 1 month) |
| Shelf Life | 24 months at -20°C |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Potency verification by antimicrobial assay, sterility, endotoxin <0.25 EU/mL, mycoplasma negative |
| Key Features | Standard antibiotic supplement for cell culture; sterile 100x concentrate; broad spectrum; validated for mammalian cell compatibility; cost effective |
For research use only, not for clinical use.
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