- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | PBCV DNA Ligase for RNA-Splinted Ligation and Target Enrichment (25 U/uL) |
| Catalog No. | IVDRM-0431 |
| Description | Also known as SplintR ligase or Chlorella virus DNA ligase, this ATP dependent enzyme from phage PBCV-1 efficiently joins two adjacent DNA strands hybridised to a complementary RNA splint, with the acceptor strand providing the 3 prime hydroxyl and the donor strand the 5 prime phosphate. Its affinity for RNA splinted DNA substrates, apparent Km of 1 nM, far exceeds conventional T4 DNA ligase and enables sub nanomolar detection of distinct RNA species in complex mixtures. Established applications include padlock probe circularisation for in situ sequencing followed by phi29 driven rolling circle amplification, adjacent DNA ligation and target enrichment pipelines in next generation sequencing and molecular diagnostics. |
| Intended Use | Padlock probe circularisation for in situ sequencing, RNA splinted ligation, RNA detection and target enrichment for NGS and molecular diagnostics |
| Principle / Technology | ATP dependent ligation of adjacent DNA strands on a complementary RNA splint |
| Performance Range / Specifications | Apparent Km of 1 nM on RNA splinted DNA substrates; sub nanomolar RNA detection sensitivity |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1250 U; 5000 U |
| Quality Control | Free of exonuclease, endonuclease and RNase residues; phosphatase below 0.0001 umol/min |
| Key Features | Far higher activity than T4 DNA ligase on RNA splinted substrates; suits in situ sequencing and target enrichment |
| Purity | Greater than 95% |
| Concentration | 25 U/uL |
| Readouts / Downstream Assays | Ligation of splinted DNA; padlock probe circularisation and RCA readout |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
