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| Product Name | PacI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0450 |
| Description | Recognising the AT rich octameric site 5 prime TTAAT^TAA 3 prime, this rapid recombinant endonuclease acts as a rare cutter that releases large inserts with minimal internal cleavage. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 10 U/uL, and runs in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. EcoKI methylation possibly overlapping the site may affect cutting, and no alternative named isoschizomers are documented. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Rare cutting for release of large inserts and rapid DNA digestion |
| Principle / Technology | Site specific cleavage at 5 prime TTAAT^TAA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoKI methylation may affect cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | Rare AT rich octamer cutter; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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