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Organoid Cell Recovery Solution

Cat.No: RESK-0203 Datasheet

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Product Details Related Products
Product Name Organoid Cell Recovery Solution
Catalog No. RESK-0203
Description Organoid Cell Recovery Solution is designed for use during observation, assay, and passaging of organoids cultured in extracellular matrix (ECM). Its proprietary components enable organoids to be released from the matrix gel and dissociated into small cell clusters or single cells. The entire process is gentle and rapid while maintaining cell viability. The product contains no biological enzymes.
Intended Use For the recovery, dissociation, passaging, and cryopreservation preparation of organoids cultured in extracellular matrix-based 3D systems.
Principle / Technology An enzyme-free, gentle chemical dissociation approach that depolymerizes the matrix gel and releases organoids as small cell clusters or single cells without compromising cell viability.
Sample Type Organoids cultured in extracellular matrix (ECM) gels.
Performance Range / Specifications Gentle conditions that do not damage organoids
Suitable for organoid passaging and cryopreservation
Reaction Conditions / Protocol If the matrix gel is difficult to dissolve: wash the organoid-gel culture twice with PBS, then add the recovery solution and digest at 4 °C for 45 min, shaking several times during incubation.
Components / Formulation Enzyme-free recovery solution containing proprietary components; contains no biological enzymes.
Storage Conditions Store at 2-8 °C.
Shelf Life 1 year when stored at 2-8 °C.
Package Specifications 100 mL
Product Form Liquid solution
Shipping Conditions Shipped with ice packs.
Compatibility Compatible with extracellular matrix (ECM) gel-based organoid culture systems.
Application Notes / Precautions For dense matrix gels, pre-wash with PBS twice before adding the recovery solution to improve digestion efficiency.

For research use only, not for clinical use.

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