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| Product Name | Organoid Cell Freezing Medium |
| Catalog No. | RESK-0204 |
| Description | Organoid Cell Freezing Medium is a ready-to-use dedicated reagent with a unique formulation that combines high efficiency, low toxicity, and convenience, designed specifically for ultra-low-temperature long-term preservation of organoids. It maximally preserves the structural integrity, proliferative activity, and functional phenotype of organoids after thawing, with a stable post-thaw viability of >=80%, meeting the needs of long-term organoid preservation. No on-site preparation is required. The medium is compatible with commonly used matrix gels and 3D culture systems, contains no animal-derived components, and reduces the risk of bacterial, viral, and mycoplasma contamination. It is suitable for basic life science research, precision oncology, drug screening, and organoid biobanking. |
| Intended Use | For the long-term cryopreservation of organoids and cell lines of various mammalian origins in ultra-low-temperature storage, including organoid biobanking, drug screening, and precision medicine research. |
| Principle / Technology | A proprietary cryoprotective formulation that protects organoids from freezing damage during controlled-rate cooling and ultra-low-temperature storage, preserving structural integrity, proliferative activity, and functional phenotype upon thawing. |
| Sample Type | Organoids of various mammalian origins and cell lines. |
| Performance Range / Specifications | Unique formulation with stable post-thaw organoid viability >=80%, effectively preserving structural integrity, proliferative activity, and functional phenotype Suitable for long-term low-temperature preservation of organoids and cell lines from various mammalian sources Free of animal-derived proteins, reducing the risk of bacterial, viral, and mycoplasma contamination and ensuring sample safety |
| Reaction Conditions / Protocol | Freezing: Take well-growing organoids (e.g., mouse small intestinal/pancreatic organoids), wash with PBS, centrifuge, and discard the supernatant. Resuspend in this freezing medium (approximately 5 x 10^4 organoids per mL of freezing medium). Aliquot and place in a controlled-rate freezing container, cooling at -1 °C/min. Keep at -80 °C for 24 h, then transfer to liquid nitrogen for long-term storage. Thawing: Thaw rapidly in a 37 °C water bath (1-2 min), then wash thoroughly to completely remove residual freezing medium. Storage of thawed medium: After thawing, keep on ice or at 4 °C and use within 24 h. |
| Components / Formulation | Ready-to-use proprietary formulation; free of animal-derived components; contains cryoprotective agents. |
| Storage Conditions | Store at -25 to -15 °C, protected from light and tightly sealed. After thawing, store on ice or at 4 °C and use within 24 h. Aliquot after the first thaw to avoid repeated freeze-thaw cycles. |
| Shelf Life | 24 months when stored at -25 to -15 °C protected from light. |
| Package Specifications | 100 mL |
| Product Form | Ready-to-use liquid medium |
| Quality Control | Validated by organoid freezing and recovery testing; post-thaw viability stably >=80%. |
| Source / Origin | Animal-component-free formulation (no animal-derived proteins). |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Valid for 24 months at -25 to -15 °C. Validated long-term performance: mouse small intestinal/pancreatic organoids cryopreserved for 1, 3, and 6 months showed post-thaw viabilities of 88%, 85%, and 82%, respectively, with intact morphology, clear edges, and no obvious fragmentation. Growth resumed 48 h after plating, with evident organoid growth at 72 h, comparable to freshly cultured organoids in proliferation rate and morphology. |
| Compatibility | Compatible with commonly used matrix gels and 3D culture systems; suitable for organoids and cell lines of various mammalian origins. |
| Application Notes / Precautions | Do not subject to repeated freeze-thaw cycles, which destroy cryoprotective components; aliquot for single use. Use well-growing organoids for freezing; strictly follow the -1 °C/min controlled-rate cooling program; thaw rapidly in a 37 °C water bath (1-2 min) and wash away residual freezing medium thoroughly. Cloudiness or precipitate usually results from improper storage temperature, repeated freeze-thaw, or product expiration. Slight cloudiness can be mixed before use; if cloudiness is severe or substantial precipitate appears, discontinue use. For rare or special organoid types, a small-scale pilot experiment is recommended before first use. If thawed organoids are morphologically intact but fail to proliferate, optimize the post-thaw culture system (medium suitability, matrix gel quality, seeding density, incubator conditions) rather than the freezing step. |
For research use only, not for clinical use.
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