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| Product Name | Nt.BstNBI Nicking Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0540 |
| Description | A nicking endonuclease that cuts only one strand of a double stranded DNA substrate, Nt.BstNBI recognises 5 prime GAGTCNNNN^N 3 prime and introduces a single-strand nick without severing the duplex, which suits in vitro DNA manipulation such as nick generation followed by labelling or cloning. The enzyme runs in its dedicated buffer at 55°C and is heat inactivated at 80°C for 20 minutes, and one unit converts 1 microgram of supercoiled pUC19 DNA completely to the open circular form in one hour at 55°C in a 50 microlitre reaction. Cleavage is unaffected by substrate methylation. |
| Intended Use | Nick generation in double stranded DNA for downstream labelling and cloning |
| Principle / Technology | Single strand cleavage at the 5 prime GAGTCNNNN^N 3 prime site leaving the duplex intact |
| Sample Type | Supercoiled plasmid DNA containing the recognition site |
| Specificity | Nicks one strand only; unaffected by substrate methylation |
| Reaction Conditions / Protocol | Digest in 1x dedicated buffer at 55°C; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2000 U |
| Key Features | Methylation insensitive nicking at 55°C |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Conversion of supercoiled substrate to open circular form |
For in vitro diagnostic use.
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