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| Product Name | NsiI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0447 |
| Description | Cutting 5 prime ATGCA^T 3 prime to leave a 3 prime overhang, this rapid recombinant endonuclease digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It is supplied at 20 U/uL, operates in the unified rapid series buffer at 37°C, and is heat inactivated at 80°C for 20 minutes. EcoBI methylation overlapping the sequence may influence cutting. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime ATGCA^T 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation overlapping the sequence may affect cleavage |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | Single shared buffer across the rapid series; activity redundancy for difficult templates |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Digestion profile on agarose gel |
For in vitro diagnostic use.
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