- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | NSC-27 Serum-Free Supplement without Vitamin A, Animal Origin-Free (50x) |
| Catalog No. | RESK-0214 |
| Description | NSC-27 Serum-Free Supplement without Vitamin A, Animal Origin-Free (50x) is an optimized serum-free supplement formulation for neuronal cell culture, manufactured entirely without animal-derived components and with vitamin A removed. Supplied as a 50x concentrated working solution, it supports the growth and maintenance of embryonic, postnatal and adult hippocampal neurons and other central nervous system (CNS) neurons when combined with a neuronal basal medium, without the need for feeder layer cells. Removal of vitamin A prevents the differentiation of neural stem cells into neurons, making this variant suitable for neural stem cell maintenance and expansion. |
| Intended Use | Used in combination with a neuronal basal medium for serum-free neuronal culture without feeder layers. The vitamin A-free formulation prevents vitamin A-induced differentiation of neural stem cells into neurons and is recommended for neural stem cell culture. For research use only. |
| Principle / Technology | An optimized, serum-free and animal origin-free neuronal culture supplement formulation lacking vitamin A; vitamin A (retinoic acid precursor) removal prevents spontaneous neuronal differentiation of neural stem cells while still supporting cell growth and survival. |
| Reaction Conditions / Protocol | Add the 50x supplement to neuronal basal medium at a 1:50 dilution to prepare complete culture medium. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 2 years when stored at -25 to -15 °C. Once thawed, keep at 4 °C for no more than 1 week or at room temperature for no more than 48 hours. |
| Package Specifications | 1 mL 10 mL |
| Product Form | Liquid, 50x concentrate |
| Quality Control | Stringent quality control; strictly sterile and endotoxin-free; high culture performance. |
| Source / Origin | Animal origin-free formulation; contains no animal-derived components. Vitamin A removed. |
| Shipping Conditions | Shipped frozen. |
| Expiration Date / Stability | Stable for 24 months at -30 to -5 °C; at 4 °C use within 1 week; at room temperature use within 48 hours. |
| Compatibility | Compatible with neuronal basal media for serum-free neuronal and neural stem cell culture. |
| Recommended Buffer System | Dilute into a neuronal basal medium. |
| Application Notes / Precautions | Use this vitamin A-free variant when maintaining neural stem cells in an undifferentiated state; the vitamin A-containing standard formulation is preferred for mature neuron culture. Protect from light during storage. |
| Batch-to-Batch Consistency | Highly consistent between batches with minimal lot-to-lot variation. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
