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| Product Name | NSC-27 Serum-Free Supplement without Vitamin A (50x) |
| Catalog No. | RESK-0210 |
| Description | NSC-27 Serum-Free Supplement without Vitamin A (50x) is a vitamin A-free version of the widely cited NSC-27 neuronal cell culture supplement, an optimized serum-free additive used to support the growth and viability maintenance of embryonic, postnatal and adult hippocampal neurons and other central nervous system (CNS) neurons. Supplied as a 50x concentrate, it is used in combination with neuronal basal medium for serum-free neuronal culture without the need for feeder layer cells. Removal of vitamin A helps prevent the differentiation of neural stem cells into neurons, making this formulation suitable for applications where maintenance of the neural stem cell state is desired. |
| Intended Use | Serum-free culture of embryonic, postnatal and adult hippocampal neurons and other CNS neurons. Culture of neural stem cells where prevention of vitamin A-induced differentiation into neurons is required. For research use only. |
| Principle / Technology | An optimized serum-free neuronal culture supplement formulation with vitamin A removed, so that neural stem cells are maintained without vitamin A-driven differentiation toward neuronal lineages. |
| Sample Type | Embryonic, postnatal and adult hippocampal neurons; other central nervous system (CNS) neurons; neural stem cells |
| Performance Range / Specifications | Optimized serum-free supplement for neuronal culture. Vitamin A removed to prevent differentiation of neural stem cells into neurons. Manufactured under stringent quality control. |
| Components / Formulation | A defined mixture of vitamins, antioxidants, hormones and other supplements formulated for serum-free neuronal culture, with vitamin A removed. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 2 years when stored at -25 to -15 °C, protected from light. |
| Package Specifications | 10 mL 1 mL |
| Product Form | Liquid, 50x concentrate |
| Quality Control | Manufactured under stringent quality control to ensure lot-to-lot performance. |
| Concentration | 50x concentrate; dilute 1:50 into neuronal basal medium before use. |
| Shipping Conditions | Ship on dry ice or at frozen temperature; store at -25 to -15 °C upon receipt. |
| Expiration Date / Stability | Stable for 24 months at -25 to -15 °C. After thawing, keep at 2-8 °C for no more than 1 week or at room temperature for no more than 48 hours. |
| Compatibility | Designed for use with neuronal basal medium for serum-free neuronal culture without feeder layer cells. |
| Recommended Buffer System | Dilute into neuronal basal medium at a 1:50 ratio. |
| Application Notes / Precautions | Vitamin A has been removed from this formulation to prevent differentiation of neural stem cells into neurons. After thawing, store at 2-8 °C for no more than 1 week; keep at room temperature for no more than 48 hours. Protect from light during storage. For research use only. |
| Batch-to-Batch Consistency | Stringent quality control ensures consistent performance between batches. |
For research use only, not for clinical use.
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