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| Product Name | NSC-27 Serum-Free Supplement without Antioxidant (50x) |
| Catalog No. | RESK-0211 |
| Description | NSC-27 Serum-Free Supplement without Antioxidant (50x) is an optimized serum-free supplement formulation for neuronal cell culture, supplied as a 50x concentrated working solution. It supports the growth and long-term viability of embryonic, postnatal and adult hippocampal neurons as well as other central nervous system (CNS) neurons. In this variant, the antioxidant components have been removed to eliminate interference from antioxidants in studies of oxidative stress and injury, oxygen free radical-induced neuronal damage, and apoptosis. |
| Intended Use | Used in combination with a neuronal basal medium for serum-free culture of prenatal, embryonic, postnatal and adult CNS neurons without feeder layers. The antioxidant-free formulation is particularly suited for research on oxidative stress, free radical-mediated neuronal injury and apoptosis. For research use only. |
| Principle / Technology | An optimized, serum-free neuronal culture supplement formulation that provides the nutrients required for neuronal growth and survival when added to a basal medium, eliminating the need for serum or feeder layer cells. |
| Reaction Conditions / Protocol | Add the 50x supplement to neuronal basal medium at a 1:50 dilution to prepare complete culture medium. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 2 years when stored at -25 to -15 °C. Once thawed, keep at 4 °C for no more than 1 week or at room temperature for no more than 48 hours. |
| Package Specifications | 1 mL 10 mL |
| Product Form | Liquid, 50x concentrate |
| Quality Control | Manufactured under stringent quality control; optimized serum-free formulation. |
| Source / Origin | Chemically formulated supplement; antioxidant components removed. |
| Shipping Conditions | Shipped frozen. |
| Expiration Date / Stability | Stable for 24 months at -30 to -5 °C; at 4 °C use within 1 week; at room temperature use within 48 hours. |
| Compatibility | Compatible with neuronal basal media for serum-free neuronal culture. |
| Recommended Buffer System | Dilute into a neuronal basal medium. |
| Application Notes / Precautions | The antioxidant-free formulation should be selected when antioxidants would interfere with the experimental readout, e.g., in oxidative stress or free radical injury models. Protect from light during storage. |
For research use only, not for clinical use.
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