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| Product Name | NSC-27 Serum-Free Supplement, Animal Origin-Free (50x) |
| Catalog No. | RESK-0213 |
| Description | NSC-27 Serum-Free Supplement, Animal Origin-Free (50x) is an optimized serum-free supplement formulation for neuronal cell culture, manufactured entirely without animal-derived components. Supplied as a 50x concentrated working solution, it supports the growth and long-term viability of embryonic, postnatal and adult hippocampal neurons and other central nervous system (CNS) neurons. When added to a neuronal basal medium, it enables neuronal culture without feeder layer cells, and supports prenatal and embryonic neuron culture with optimized viability and long-term survival. |
| Intended Use | Used in combination with a neuronal basal medium for serum-free culture of prenatal, embryonic, postnatal and adult CNS neurons without feeder layers. The animal origin-free formulation is suited for applications requiring animal component-free culture systems. For research use only. |
| Principle / Technology | An optimized, serum-free and animal origin-free neuronal culture supplement formulation that provides the nutrients required for neuronal growth and survival when added to a basal medium, eliminating the need for serum or feeder layer cells. |
| Reaction Conditions / Protocol | Add the 50x supplement to neuronal basal medium at a 1:50 dilution to prepare complete culture medium. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 2 years when stored at -25 to -15 °C. Once thawed, keep at 4 °C for no more than 1 week or at room temperature for no more than 48 hours. |
| Package Specifications | 1 mL 10 mL |
| Product Form | Liquid, 50x concentrate |
| Quality Control | Stringent quality control; strictly sterile and endotoxin-free; high culture performance. |
| Source / Origin | Animal origin-free formulation; contains no animal-derived components. |
| Shipping Conditions | Shipped frozen. |
| Expiration Date / Stability | Stable for 24 months at -30 to -5 °C; at 4 °C use within 1 week; at room temperature use within 48 hours. |
| Compatibility | Compatible with neuronal basal media for serum-free neuronal culture. |
| Recommended Buffer System | Dilute into a neuronal basal medium. |
| Application Notes / Precautions | Protect from light during storage. Choose the animal origin-free version for culture systems where animal-derived components must be excluded. |
| Batch-to-Batch Consistency | Highly consistent between batches with minimal lot-to-lot variation. |
For research use only, not for clinical use.
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