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| Product Name | NSC-27 Serum-Free Supplement (50x) |
| Catalog No. | RESK-0209 |
| Description | NSC-27 Serum-Free Supplement (50x) is one of the most widely cited neuronal cell culture supplements. It is an optimized serum-free additive used to support the growth and viability maintenance of embryonic, postnatal and adult hippocampal neurons and other central nervous system (CNS) neurons. Supplied as a 50x concentrate, it is used in combination with neuronal basal medium for serum-free neuronal culture without the need for feeder layer cells. In neuronal basal medium, NSC-27 supplement supports the culture of prenatal and embryonic neurons with optimized viability and long-term survival. A vitamin A-free version and an antioxidant-free version of this supplement are also available: the vitamin A-free formulation helps prevent differentiation of neural stem cells into neurons, while the antioxidant-free formulation avoids interference from antioxidants in studies of oxidative stress, oxidative injury, and free radical-mediated neuronal damage and apoptosis. |
| Intended Use | Serum-free culture of embryonic, postnatal and adult hippocampal neurons and other CNS neurons. Supports prenatal and embryonic neuron culture with optimized viability and long-term survival. For research use only. |
| Principle / Technology | An optimized serum-free neuronal culture supplement formulation that replaces serum and feeder layer requirements for neuronal cell culture. |
| Sample Type | Embryonic, postnatal and adult hippocampal neurons; other central nervous system (CNS) neurons; neural stem cells |
| Performance Range / Specifications | Optimized serum-free supplement for neuronal culture. Supports optimized viability and long-term survival of prenatal and embryonic neurons. Manufactured under stringent quality control. |
| Components / Formulation | A defined mixture of vitamins, antioxidants, hormones and other supplements formulated for serum-free neuronal culture. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 2 years when stored at -25 to -15 °C. |
| Package Specifications | 10 mL 1 mL |
| Product Form | Liquid, 50x concentrate |
| Quality Control | Manufactured under stringent quality control to ensure lot-to-lot performance. |
| Concentration | 50x concentrate; dilute 1:50 into neuronal basal medium before use. |
| Shipping Conditions | Ship on dry ice or at frozen temperature; store at -25 to -15 °C upon receipt. |
| Expiration Date / Stability | Stable for 24 months at -25 to -15 °C. After thawing, keep at 2-8 °C for no more than 1 week or at room temperature for no more than 48 hours. |
| Compatibility | Designed for use with neuronal basal medium for serum-free neuronal culture without feeder layer cells. |
| Recommended Buffer System | Dilute into neuronal basal medium at a 1:50 ratio. |
| Application Notes / Precautions | The supplement is a complex mixture of multiple components at different concentrations; it cannot be directly converted to a single mg/mL value. After thawing, store at 2-8 °C for no more than 1 week; keep at room temperature for no more than 48 hours. For research use only. |
| Batch-to-Batch Consistency | Stringent quality control ensures consistent performance between batches. |
For research use only, not for clinical use.
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