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| Product Name | NruI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0446 |
| Description | Recognising 5 prime TCG^CGA 3 prime, this rapid recombinant endonuclease prepares DNA fragments and cuts plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it runs in the shared rapid series buffer at 37°C CpG methylation fully overlapping the site blocks cleavage. Note that the enzyme cannot be heat inactivated; remove it after digestion by phenol chloroform extraction or column purification. |
| Intended Use | Rapid DNA fragment preparation for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime TCG^CGA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Single shared buffer across the rapid series; activity redundancy for excess substrate |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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