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| Product Name | NotI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0445 |
| Description | Recognising the GC rich octameric site 5 prime GC^GGCCGC 3 prime, this rapid recombinant endonuclease is a rare cutter valued for releasing intact inserts from vectors with minimal internal cutting. It rapidly digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and performs in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, an important consideration for methylated genomic DNA, and CciNI is the documented isoschizomer. |
| Intended Use | Rapid digestion for molecular cloning where rare cutting preserves large inserts intact |
| Principle / Technology | Site specific cleavage at 5 prime GC^GGCCGC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; isoschizomer CciNI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Rare GC rich octamer cutter; single shared buffer across the rapid series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Gel profile of digested DNA |
For in vitro diagnostic use.
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