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| Product Name | NLS-Cas9-EGFP Nuclease, High Concentration (10 mg/mL) |
| Catalog No. | IVDRM-0587 |
| Description | A recombinant nuclease that fuses a nuclear localisation signal, Streptococcus pyogenes Cas9 and enhanced green fluorescent protein, built for high efficiency DNA-free CRISPR editing. The N-terminus carries the nuclear localisation signal while the C-terminus carries EGFP and a 6-His purification tag, so once the Cas9 ribonucleoprotein complex enters the cell the signal drives rapid nuclear localisation without any transcription or translation step and raises editing efficiency. The EGFP tag marks transfected cells for real-time tracking and fluorescence-activated cell sorting, allowing edited populations to be enriched and trimming the downstream workload and cost of single-cell cloning and genotyping. Purity is at least 95% with no endonuclease, exonuclease or RNase activity. |
| Intended Use | DNA-free CRISPR editing with fluorescent tracking and sorting of successfully edited cells |
| Principle / Technology | NLS-mediated nuclear localisation with EGFP tagging for real-time tracking of Cas9 ribonucleoprotein editing |
| Sample Type | Cells transfected with Cas9 ribonucleoprotein complexes |
| Specificity | Nuclear localisation signal, EGFP and 6xHis tags; endonuclease, exonuclease and RNase activity negative |
| Reaction Conditions / Protocol | Transfect the ribonucleoprotein complex and enrich edited cells by fluorescence-activated cell sorting |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 ug; 1 mg |
| Key Features | Fluorescent tracking and sorting of edited cells; no vector or transcription step needed |
| Purity | Greater than or equal to 95% |
| Concentration | 10 to 12 mg/mL |
| Readouts / Downstream Assays | Fluorescence imaging and sorting efficiency with an editing readout at the target locus |
For in vitro diagnostic use.
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