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| Product Name | NK Cell Serum-Free Culture Medium |
| Catalog No. | RESK-0488 |
| Description | NK Cell Serum-Free Culture Medium is an expansion culture kit specifically designed for NK cell culture. It is serum-free, free of xenogeneic animal-derived components, and manufactured with pharmaceutical-grade raw and auxiliary materials. The medium is suitable for the in vitro activation and expansion of NK cells from autologous peripheral blood or umbilical cord blood PBMCs, yielding NK cells of high purity. |
| Intended Use | In vitro activation and expansion of NK cells from autologous peripheral blood or umbilical cord blood PBMCs to obtain high-purity NK cells. |
| Sample Type | Peripheral blood or umbilical cord blood PBMCs |
| Performance Range / Specifications | 1. Serum-free and free of xenogeneic components. 2. Supports robust cell proliferation with high viability, high expansion and high purity. Culture of PBMCs from healthy donors with this kit demonstrates high NK cell expansion and high cell viability. |
| Reaction Conditions / Protocol | 1. Sample requirements: 1) Peripheral blood: PBMC > 3 x 10^7 cells (recommended blood volume approximately 50 mL collected in sodium heparin vacuum tubes). Processing within 4 hours after blood collection and lymphocyte subset analysis are recommended. Cryopreserved peripheral blood is not recommended. 2) Umbilical cord blood (without anticoagulant): recommended collection volume > 50 mL. It is recommended to withdraw at least half of the anticoagulant (approximately 14 mL) from the collection bag; for other containers, the anticoagulant should not exceed 14% (or PBMC > 3 x 10^7 cells). Process within 12 hours after collection. 2. Seeding density: the recommended initial PBMC seeding density is 2 x 10^6 cells/mL; a higher initial seeding density may be used for cord blood. 3. Feeding density: cell density before feeding is generally 1.5-2 x 10^6 cells/mL; cell density after feeding is generally 0.6-1 x 10^6 cells/mL and must not be lower than 0.6 x 10^6 cells/mL. 4. Medium use: allow the medium to warm naturally to room temperature before each feeding; prepare the expansion medium (containing IL-2) fresh and use within one week. |
| Components / Formulation | NK cell serum-free medium plus NK cell supplements A-E; serum-free, free of xenogeneic animal-derived components; manufactured with pharmaceutical-grade raw and auxiliary materials. |
| Storage Conditions | NK cell supplements A-E: store at -25 to -15 °C, protected from light. NK cell serum-free medium: store at 2-8 °C, protected from light. |
| Shelf Life | 1 year (both medium and supplements A-E). |
| Package Specifications | 2000 mL |
| Product Form | Liquid medium with frozen supplements |
| Expiration Date / Stability | 1. NK cell serum-free medium: 1 year at 2-8 °C protected from light. 2. NK cell supplements A-E: 1 year at -25 to -15 °C protected from light. 3. Prepared expansion medium (containing IL-2) has a short usable period; use within one week. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | 1. Allow the medium to warm naturally to room temperature before each feeding. 2. Do not place the whole bottle of medium in a 37 °C incubator for warming, as this will accelerate the inactivation of cytokines in the feeding medium. 3. The prepared expansion medium (containing IL-2) has a short usable period and should not be used beyond one week, especially during the early activation phase (the first 7 days). 4. Cryopreserved peripheral blood is not recommended; process peripheral blood within 4 hours and cord blood within 12 hours after collection. |
For research use only, not for clinical use.
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