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| Product Name | NK Cell Exosomes (Human) |
| Catalog No. | RESK-0624 |
| Description | NK Cell Exosomes (Human) are high-purity exosome particles isolated from the supernatant of serum-free cultured NK cells, with a diameter of 30-150 nm. They can be used as a research reagent for transmission electron microscopy (TEM) analysis, NTA particle size analysis, nucleic acid and protein analysis, and cell-based and animal experiments. |
| Intended Use | Research reagent for TEM morphological observation, NTA particle size analysis, exosome nucleic acid/protein composition analysis, and exosome functional studies in cell-based and animal experiments. |
| Principle / Technology | Purified by tangential flow filtration combined with chromatography. |
| Detection Method | Transmission electron microscopy (TEM), NTA particle size analysis, nucleic acid and protein analysis, Western Blot (CD63 and CD9 detection). |
| Sample Type | Purified exosome preparation (ready-to-use exosome standard). |
| Performance Range / Specifications | Particle size: 30-150 nm Purity: high purity Package content: 100 ug per vial, supplied as a liquid with a fill volume of approximately 60 uL per vial (the exact volume varies slightly with batch-to-batch concentration differences). Typical yield reference: approximately 3 mg of exosomes can be extracted per 1 L of cell culture supernatant; because the finished exosomes undergo a concentration step with substantial loss, direct conversion is not possible and the actual yield should be evaluated from experimental data. |
| Storage Conditions | -80 °C. |
| Shelf Life | 9 months at -80 °C. |
| Package Specifications | 100 ug |
| Product Form | Liquid (aqueous exosome preparation), approximately 60 uL per vial. |
| Purity | High purity. |
| Concentration | 100 ug per vial in approximately 60 uL; the exact fill volume varies slightly between batches due to concentration differences. |
| Source / Origin | Isolated from the supernatant of serum-free cultured NK cells; human origin. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | Fully lyse the product before Western Blot detection; an exosome-specific lysis buffer is recommended. For Western Blot detection of exosome markers (e.g., CD63 and CD9), add 1/4 volume of 5x SDS-PAGE protein loading buffer and heat at 100 °C for 10 min; for multi-pass transmembrane proteins such as CD9, heating at 70 °C for 10 min is recommended to avoid protein aggregation or denaturation. |
| Heat Inactivation | Not applicable; for WB sample preparation, heat at 100 °C for 10 min (70 °C for 10 min for multi-pass transmembrane proteins such as CD9). |
For research use only, not for clinical use.
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