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| Product Name | NheI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0444 |
| Description | Recognising 5 prime G^CTAGC 3 prime, this rapid recombinant endonuclease generates cohesive ends compatible with SpeI and XbaI digests, a property widely used in multi fragment assembly schemes such as the Gibson-independent, golden-gate-style assembly strategies. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and operates in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation possibly overlapping the site obstructs cleavage, and AsuNHI is the documented isoschizomer. |
| Intended Use | DNA cutting and molecular cloning with compatible cohesive end strategies |
| Principle / Technology | Site specific cleavage at 5 prime G^CTAGC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation possibly overlapping the site blocks cleavage; isoschizomer AsuNHI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | Cohesive ends compatible with SpeI and XbaI for assembly cloning; single shared buffer across the series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion fragment pattern on agarose gel |
For in vitro diagnostic use.
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