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| Product Name | NF-κB (Luc) HEK293 Reporter Cell |
| Catalog No. | ATR-CCR-0063 |
| Description | An adherent HEK293 reporter cell line engineered with an NF-κB response element controlling luciferase expression. Pathway activation produces a measurable luminescent signal for signaling bioassays. |
| Intended Use | For discovery and evaluation of NF-κB pathway activators or inhibitors and as a transfection host for receptors associated with NF-κB signaling. |
| Principle / Technology | Ligand-dependent receptor activation triggers intracellular signaling and NF-κB response element-driven luciferase expression, producing a luminescent readout. |
| Detection Method | Luciferase luminescence signaling bioassay |
| Sample Type | Stable mammalian reporter cell line |
| Performance Range / Specifications | Approximately 5 × 10^6 cells in 1 mL per vial; human TNF-α EC50 approximately 0.9507 ng/mL; maximum induction approximately 611-fold; stable response reported across passages 9-30. |
| Sensitivity / LOD | Human TNF-α EC50: approximately 0.9507 ng/mL |
| Specificity | NF-κB pathway activation |
| Reaction Conditions / Protocol | Culture in DMEM with 10% FBS and 100 μg/mL hygromycin. Stimulate with the relevant pathway ligand and quantify luciferase-dependent luminescence. |
| Components / Formulation | Cryopreservation medium: 90% FBS and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80°C. |
| Package Specifications | 1 vial; approximately 5 × 10^6 cells in 1 mL |
| Product Form | Cryopreserved reporter cell line |
| Quality Control | Response to human TNF-α is characterized by relative luminescence, induction fold and passage-stability analysis. |
| Key Features | NF-κB response element-driven luciferase; hygromycin resistance; strong TNF-α response; passage-stable signaling performance; suitable for agonist and inhibitor screening. |
| Activity / Unit Definition | Maximum induction: approximately 611-fold in response to human TNF-α. |
| Source / Origin | HEK293 cells carrying an NF-κB response element-driven luciferase reporter system. |
| Expiration Date / Stability | Passage-stable signaling response reported from passages 9 through 30. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | NF-κB signaling bioassays; ligand-response testing; compound screening; receptor transfection studies |
| Recommended Buffer System | DMEM with 10% FBS and 100 μg/mL hygromycin |
| Application Notes / Precautions | Maintain hygromycin selection during routine culture and confirm assay performance with an appropriate positive-control ligand. |
| Genotype | NF-κB response element-luciferase reporter; hygromycin-resistant. |
| Alternative Names / Synonyms | NF-kB Luc HEK293 Reporter Cell; NF-κB Luciferase Reporter Cell |
| Host Species | Human |
| Tag Information | Tag-Free |
For research use only, not for clinical use.
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