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| Product Name | Neuronal Basal Medium (without L-Glutamine) |
| Catalog No. | RESK-0640 |
| Description | Neuronal Basal Medium is a basal medium developed specifically to meet the particular requirements of neural cell culture and is suitable for the culture of prenatal and embryonic neurons. When used with appropriate neuronal supplements (e.g., N-2 supplement), it supports the culture of hippocampal neurons and neural cells from the cerebral cortex and other brain regions. Its key advantage is the ability to maintain neuronal growth and normal phenotype over short- or long-term periods without a glial feeder layer, enabling the establishment of high-purity neuronal culture systems. This product is prepared with water for injection and sterilized by 0.1 um filtration. Contains: D-glucose, HEPES, phenol red and sodium pyruvate. Without: L-glutamine, L-glutamic acid and L-aspartic acid; L-glutamine must be added by the user as required. |
| Intended Use | For the culture of prenatal and embryonic neurons and neural cells, including CNS neural stem cells, neural progenitors, neuropithelial cells, and neurons of the hippocampus, striatum, substantia nigra, septum, cortex, retina, brain stem and cerebellum (granule cells, Purkinje cells), as well as GABAergic, glutamatergic and dopaminergic neurons; also suitable for glial cells (astrocytes, oligodendrocytes, radial and other specialized glia, microglia), PNS neurons (DRG dorsal root neurons, sympathetic and parasympathetic ganglia, enteric neurons), Schwann cells, satellite cells, placode cranial ganglia (auditory and optic), brain tumor cell lines and immortalized cell lines. |
| Performance Range / Specifications | Designed specifically for neural cell culture; suitable for prenatal and embryonic neurons; maintains short- or long-term neuronal growth and normal phenotype without a glial feeder layer. Sterilized by 0.1 um filtration. |
| Components / Formulation | Amino Acids Glycine 30 mg/L (0.3996 mM) L-Alanine 2 mg/L (0.0224 mM) L-Arginine hydrochloride 84 mg/L (0.3987 mM) L-Asparagine monohydrate 0.83 mg/L (0.0055 mM) L-Cysteine hydrochloride monohydrate 45.7 mg/L (0.2602 mM) L-Histidine monohydrochloride hydrate 42 mg/L (0.2004 mM) L-Isoleucine 105 mg/L (0.8005 mM) L-Leucine 105 mg/L (0.8005 mM) L-Lysine hydrochloride 146 mg/L (0.7993 mM) L-Methionine 30 mg/L (0.2011 mM) L-Serine 42 mg/L (0.3997 mM) L-Phenylalanine 66 mg/L (0.3995 mM) L-Proline 7.76 mg/L (0.0674 mM) L-Threonine 95 mg/L (0.7975 mM) L-Tryptophan 16 mg/L (0.0783 mM) L-Tyrosine disodium salt dihydrate 104 mg/L (0.3982 mM) L-Valine 94 mg/L (0.8024 mM) Vitamins Choline chloride 4 mg/L (0.0286 mM) D-Calcium pantothenate 4 mg/L (0.0168 mM) Folic Acid 4 mg/L (0.0091 mM) Niacinamide 4 mg/L (0.0328 mM) Pyridoxine hydrochloride 4 mg/L (0.0195 mM) Riboflavin 0.4 mg/L (0.0011 mM) Thiamine hydrochloride 4 mg/L (0.0119 mM) Vitamin B12 0.0068 mg/L (0.000005 mM) i-Inositol 7.2 mg/L (0.0400 mM) Inorganic Salts Calcium Chloride 200 mg/L (1.8021 mM) Ferric Nitrate nonahydrate 0.1 mg/L (0.0002 mM) Magnesium Chloride hexahydrate 165 mg/L (0.8116 mM) Potassium Chloride 400 mg/L (5.3655 mM) Sodium Bicarbonate 2200 mg/L (26.1874 mM) Sodium Chloride 3000 mg/L (51.3347 mM) Sodium Phosphate monobasic hydrate 125 mg/L (0.9059 mM) Zinc Sulfate heptahydrate 0.194 mg/L (0.0007 mM) Other Components D-(+)-Glucose 4500 mg/L (24.9778 mM) HEPES 2600 mg/L (10.9106 mM) Sodium Pyruvate 25 mg/L (0.2272 mM) Phenol Red 8.1 mg/L (0.0215 mM) |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year when stored at 2-8 °C, protected from light. |
| Package Specifications | 500 mL |
| Product Form | Liquid basal medium, ready to use after addition of the required supplements |
| Quality Control | Prepared with water for injection and sterilized by 0.1 um filtration. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Requires supplementation with neuronal culture supplements (e.g., N-2 supplement) before use; L-glutamine must be added separately by the user. For glial cell culture, add the appropriate supplements as required by the cell type. |
| Application Notes / Precautions | Add L-glutamine or L-glutamate according to the cell type: L-glutamate is excitotoxic to mature neurons and must be used differentially. 1) For primary hippocampal neurons and other embryonic neurons, add 25 uM L-glutamate to the initial plating medium and discontinue its use after 4 days. 2) For neuroblastoma, the seeding and replacement media must contain L-glutamine solution. Pre-rinse all materials that will contact cells with complete medium to prevent neuronal adhesion to plastic surfaces of culture dishes and centrifuge tubes. Add medium dropwise to avoid osmotic shock caused by abrupt osmolarity changes. Thaw supplements overnight at 4 °C or for 2 hours at room temperature; direct thawing in a 37 °C water bath is not recommended. For low neuronal survival after cryopreservation recovery: avoid prolonged exposure of cells to 37 °C during thawing (stop the water bath while a small amount of ice crystals remains at the bottom of the tube); pre-wet all plastic and glass consumable surfaces with medium before recovering cells; add medium dropwise to avoid osmotic shock; thaw only 1-2 tubes at a time to minimize prolonged exposure of cells in cryopreservation medium. |
For research use only, not for clinical use.
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