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| Product Name | NdeI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0443 |
| Description | Cutting 5 prime CA^TATG 3 prime, this rapid recombinant endonuclease targets a site that embeds the ATG start codon, making it a staple for positioning open reading frames in expression vector construction. It rapidly digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and runs in the common rapid series buffer at its 37°C optimum followed by heat inactivation at 80°C for 20 minutes. Dam, Dcm, CpG, EcoKI and EcoBI methylation have no effect on activity, and FauNDI is the recognised isoschizomer. |
| Intended Use | Expression vector construction and rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime CA^TATG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomer FauNDI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Start codon embedded site for open reading frame positioning; broad methylation tolerance |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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