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| Product Name | Nb.BsrDI Nicking Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0537 |
| Description | A nicking endonuclease that cuts only one strand of a double stranded DNA substrate, Nb.BsrDI introduces a nick at the 5 prime GCAATGNN 3 prime site without severing the duplex. The enzyme runs in the shared rapid series buffer at an incubation temperature of 65°C and is heat inactivated at 80°C for 20 minutes, and one unit converts 1 microgram of supercoiled pUC19 DNA completely to the open circular form in one hour at 65°C in a 50 microlitre reaction. Methylation of the substrate has no effect on cleavage. |
| Intended Use | Generation of single-strand nicks in double stranded DNA for cloning and nicking based workflows |
| Principle / Technology | Single strand cleavage at the 5 prime GCAATGNN 3 prime site leaving the duplex intact |
| Sample Type | Supercoiled plasmid DNA containing the recognition site |
| Specificity | Nicks one strand only; unaffected by substrate methylation; 65°C incubation |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 65°C; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2000 U |
| Key Features | High temperature nicking at 65°C; methylation insensitive cleavage |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Conversion of supercoiled substrate to open circular form |
For in vitro diagnostic use.
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