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| Product Name | N-2 Supplement, Serum-Free, 100X (Animal-Origin-Free) |
| Catalog No. | RERE-0025 |
| Description | N-2 is a chemically defined supplement built for serum-free neuronal culture. It is recommended for the growth and expression of post-mitotic neurons derived from the central and peripheral nervous systems and for neuroblastoma cell lines, and it pairs with a serum-free neuronal basal medium - or DMEM/F12 - to which growth factors such as bFGF and EGF have been added. Every component is defined, so the formulation stays transparent and reproducible run after run. The stock is supplied at 100X strength, sterility is tightly controlled with no endotoxin, and this version is produced entirely without animal-derived raw materials for workflows that require an animal-origin-free chain. Batch-to-batch variation is kept small so cultures behave predictably between orders. |
| Intended Use | Serum-free culture of post-mitotic neurons from CNS and PNS origins; culture of neuroblastoma cell lines; pairing with bFGF- and EGF-supplemented basal media or DMEM/F12; organoid and neuronal differentiation workflows requiring defined supplements. |
| Principle / Technology | Defined trace components keep post-mitotic neurons viable and expressive in the absence of serum, replacing the ill-defined factors serum would otherwise provide. |
| Sample Type | Sterile 100X aqueous concentrate, animal-origin-free. |
| Performance Range / Specifications | Chemically defined composition; 100X working strength; strictly sterile and endotoxin-free; animal-origin-free; small lot-to-lot variation; prompt availability from stock. |
| Specificity | Concentration: 100X; source: non-animal origin; sterility: strictly sterile; endotoxin: none detected; storage at -25 °C to -15 °C protected from light; shelf life 2 years. |
| Reaction Conditions / Protocol | Thaw on ice, dilute 1:100 into the serum-free neuronal basal medium or DMEM/F12 carrying the chosen growth factors, and mix gently before use. |
| Components / Formulation | One bottle of 100X N-2 Supplement (1 mL or 5 mL). |
| Storage Conditions | Store at -25 °C to -15 °C protected from light; a shelf life of 2 years applies. |
| Shelf Life | 2 years when stored as recommended. |
| Package Specifications | 1 mL or 5 mL per bottle |
| Product Form | Aqueous concentrate, 100X. |
| Quality Control | Released on sterility and endotoxin-free status with small lot-to-lot variation. |
| Key Features | - Chemically defined, animal-origin-free composition - Strictly sterile and endotoxin-free - Small lot-to-lot variation for reproducible cultures - Purpose-built for post-mitotic CNS/PNS neurons and neuroblastoma lines |
| Purity | Solution grade; a solid-state purity figure does not apply. |
| Concentration | 100X concentrate, dosed 1:100 into the final medium. |
| Activity / Unit Definition | Functional performance is expressed through neuronal culture support rather than a numeric activity unit. |
| Source / Origin | Chemically defined components of non-animal origin. |
| Shipping Conditions | Shipped chilled. |
| Expiration Date / Stability | Stable through the stated shelf life when stored frozen and protected from light; avoid repeated freeze-thaw. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Pairs with serum-free neuronal basal media and DMEM/F12 supplemented with growth factors such as bFGF and EGF. |
| Recommended Buffer System | Aqueous formulation; no separate buffer needed. |
| Application Notes / Precautions | Thaw on ice and mix gently, aliquot to avoid repeated freeze-thaw, and keep sterile technique throughout. |
| Batch-to-Batch Consistency | Strict release testing keeps lot-to-lot variation small. |
| Model Type | Defined serum-free medium supplement |
| Starting Material / Tissue Site | Chemically defined N-2 formulation, animal-origin-free |
| Delivery Standard | 1 mL or 5 mL bottle |
| Turnaround Time | dispatch is arranged at order confirmation. |
| Readouts / Downstream Assays | Neuronal viability, morphology and marker expression in serum-free culture. |
For research use only, not for clinical use.
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