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| Product Name | N-2 Supplement, 100x, for Neural Stem Cells |
| Catalog No. | ATR-CCR-0023 |
| Description | A 100-fold concentrated chemically defined serum-free supplement based on Bottenstein's N2 formula, containing insulin, transferrin, progesterone, putrescine, and selenite for neural stem cell and neural crest cell culture. |
| Intended Use | Supplementing DMEM/F12 medium for the serum-free culture of neural stem cells, neuroblastoma lines, and neural crest-derived cells; component of defined neuronal differentiation media for pluripotent stem cells. |
| Principle / Technology | Insulin promotes glucose uptake and cell survival signaling through IGF-1 receptor; transferrin provides iron for enzymatic function; progesterone supports myelination; putrescine promotes cell proliferation; selenite is a cofactor for glutathione peroxidase antioxidant defense. |
| Detection Method | Nestin and Sox2 expression by immunocytochemistry, neurosphere formation assay, differentiation capacity into neurons, astrocytes, and oligodendrocytes |
| Sample Type | Mouse and human neural stem cells, neuroblastoma cell lines, iPSC- and ESC-derived neural rosettes |
| Performance Range / Specifications | Maintains >95% Nestin and Sox2 expression in neural stem cell cultures; supports >5 passages in serum-free conditions |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 1 mL N-2 supplement per 100 mL DMEM/F12 medium (1:100); common to combine with 20 ng/mL EGF and 20 ng/mL bFGF for neural stem cell proliferation. |
| Components / Formulation | Human transferrin (Holotransferrin), Insulin recombinant full chain, Progesterone, Putrescine, Sodium Selenite, in DMEM/F12 base |
| Storage Conditions | -20°C (long term); 2–8°C after thawing, protect from light |
| Shelf Life | 24 months at -20°C; 4 weeks at 2–8°C in complete medium |
| Package Specifications | 5 mL, 50 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Neural stem cell proliferation assay, immunocytochemical characterization, sterility, endotoxin |
| Key Features | Chemically defined; 100x concentrate; serum-free; supports neural stem cell self-renewal; validated for mouse and human NSCs |
For research use only, not for clinical use.
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