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| Product Name | Mycoplasma Prevention Reagent (2000x) |
| Catalog No. | RESK-0517 |
| Description | Mycoplasma contamination occurs frequently during cell culture and is difficult to detect with the naked eye or microscopy, making prevention especially important. Adding a mycoplasma prevention reagent to the culture medium effectively prevents mycoplasma contamination. Because mycoplasma lacks a cell wall, conventional antibiotics are generally ineffective against it. Mycoplasma Prevention Reagent (2000x) combines commonly used antibiotics with mycoplasma-inhibitory activity in an optimized ratio; its core components are quinolone, tetracycline derivative, and macrolide antibiotics. Building on the mycoplasma elimination reagent, the concentrations of the three antibiotics have been further optimized to inhibit mycoplasma growth without harming cells. The product is supplied as a 2000x stock solution and is diluted to a 1x working concentration before use, in the same way as adding penicillin-streptomycin during cell culture. |
| Intended Use | For the prevention of mycoplasma contamination in cell culture by direct addition to the culture medium. |
| Principle / Technology | An optimized mixture of quinolone, tetracycline derivative, and macrolide antibiotics that inhibits mycoplasma growth; since mycoplasma has no cell wall, cell wall-targeting antibiotics are ineffective. |
| Reaction Conditions / Protocol | Dilute the 2000x stock solution to a 1x working concentration in the culture medium. The procedure is the same as adding penicillin-streptomycin (dual antibiotic) during routine cell culture. |
| Components / Formulation | Optimized mixture of quinolone, tetracycline derivative, and macrolide antibiotics. |
| Storage Conditions | Store at -20 °C protected from light. If not used for an extended period, keep protected from light. |
| Shelf Life | 18 months. |
| Package Specifications | 1 mL 5x 1 mL |
| Product Form | Liquid concentrate (2000x stock solution). |
| Concentration | 2000x stock solution; dilute to 1x working concentration before use. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 18 months when stored at -20 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Formulated to inhibit mycoplasma growth without harming cultured cells. |
| Application Notes / Precautions | Mycoplasma elimination and prevention reagents contain the same classes of antibiotics, differing only in the optimized formulation ratios. In most cases, an elimination reagent can be used as a prevention reagent at a reduced concentration. If an unused elimination reagent is on hand, it can be applied for prevention by halving the concentration according to the instructions in the manual. |
For research use only, not for clinical use.
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