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| Product Name | Mycoplasma Elimination Reagent (1000x) |
| Catalog No. | RESK-0516 |
| Description | During cell culture, routine testing can effectively prevent large-scale mycoplasma contamination. Once cells are contaminated, the best practice is to autoclave and discard the cells to protect other clean cell lines. However, when the contaminated cells are valuable, mycoplasma can be eliminated using a combination of antibiotics. Because mycoplasma lacks a cell wall, conventional antibiotics are generally ineffective against it. Mycoplasma Elimination Reagent (1000x) is a mixed antibiotic preparation containing three components with inhibitory activity against mycoplasma: a quinolone, a tetracycline derivative, and a macrolide antibiotic, which together achieve effective mycoplasma clearance. |
| Intended Use | For the elimination of mycoplasma contamination from cultured cells, particularly valuable or irreplaceable cell lines that cannot simply be discarded. |
| Principle / Technology | A combination of three antibiotic classes (quinolone, tetracycline derivative, and macrolide) that are active against mycoplasma, which lacks a cell wall and is therefore resistant to conventional cell wall-targeting antibiotics. |
| Specificity | Active against most mycoplasma species commonly encountered in the laboratory. |
| Reaction Conditions / Protocol | Dilute the 1000x concentrate into culture medium to a 1x working concentration. A typical elimination cycle is 1 week; for difficult-to-eliminate contamination, the treatment can be extended to 2 weeks, or the reagent can be used at a 1:500 dilution (note that this increases cytotoxicity, depending on the tolerance of the cells). |
| Components / Formulation | Mixed antibiotic preparation containing a quinolone, a tetracycline derivative, and a macrolide antibiotic. |
| Storage Conditions | Store at -20 °C protected from light. If not used for an extended period, keep protected from light. |
| Shelf Life | 18 months. |
| Package Specifications | 1 mL 5x 1 mL 100 uL |
| Product Form | Liquid concentrate (1000x). |
| Concentration | 1000x concentrate; dilute to 1x working concentration before use (1:500 for refractory contamination). |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 18 months when stored at -20 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Does not antagonize penicillin-streptomycin (dual antibiotic) in cell culture. Its components (quinolone, tetracycline derivative, and macrolide) belong to antibiotic classes distinct from penicillin and streptomycin and act through different mechanisms. |
| Application Notes / Precautions | Like a standard dual-antibiotic supplement, this reagent may have some effect on cells, but the optimized formulation minimizes cytotoxicity and causes essentially no harm to cells. It prevents and eliminates most mycoplasma species commonly found in the laboratory. If contamination occurs, discarding the cells after autoclaving is the preferred option, and the contamination source should be identified to prevent recurrence. Not suitable for long-term use; once mycoplasma has been cleared, switch to a mycoplasma prevention reagent for routine protection. The elimination reagent can also be used for prevention at a reduced concentration (half the recommended dosage, per the product manual). |
For research use only, not for clinical use.
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