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| Product Name | Murine RNase Inhibitor, High Concentration (200 U/uL) |
| Catalog No. | IVDRM-0555 |
| Description | A recombinant murine RNase inhibitor expressed and purified in soluble form from E. coli, this protein specifically inactivates ribonucleases by forming a non-covalent complex with them and broadly inhibits RNase A, RNase B and RNase C. Compared with the human inhibitor it lacks the two oxidation-sensitive cysteines of the human protein, giving higher resistance to oxidation, and it suits assays sensitive to high DTT concentrations such as qPCR. RT-PCR and RT-qPCR testing confirm compatibility with reverse transcriptases and various DNA polymerases, with no effect on the activity of SP6, T7 and T3 RNA polymerases, AMV and M-MLV reverse transcriptases and Taq DNA polymerase. Applications include first-strand cDNA synthesis, polysome isolation, in vitro transcription and cell-free translation systems where RNA must be protected. |
| Intended Use | RNase protection in first-strand cDNA synthesis, polysome isolation, in vitro transcription and cell-free translation |
| Principle / Technology | Non-covalent complex formation with RNase A, B and C that inactivates the enzymes |
| Sample Type | RNA containing reaction systems prone to RNase contamination |
| Specificity | Broad inhibition of RNase A, B and C; higher oxidation resistance than the human inhibitor; suited to high DTT sensitivity assays |
| Reaction Conditions / Protocol | Add to the reaction system at the working concentration to protect RNA templates and products |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 uL; 1 mL; 10 mL; 50 mL; 500 mL |
| Key Features | High concentration format; compatible with reverse transcriptases and polymerases without affecting their activity |
| Concentration | 200 U/uL |
| Recommended Buffer System | Hepes-KOH, KCl, DTT, non-ionic surfactants and 50% glycerol at pH 7.5 |
| Readouts / Downstream Assays | RT-qPCR performance with protected RNA templates |
For in vitro diagnostic use.
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