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| Product Name | Murine RNase Inhibitor (40 U/uL) |
| Catalog No. | IVDRM-0559 |
| Description | A recombinant murine RNase inhibitor expressed and purified in soluble form from E. coli, this protein specifically inactivates ribonucleases by binding them non-covalently into an inactive complex and broadly inhibits RNase A, RNase B and RNase C. Compared with the human inhibitor it lacks the two oxidation-sensitive cysteines of the human protein, giving higher antioxidant activity, and its low sensitivity to DTT suits high DTT sensitive assays such as qPCR. RT-PCR and RT-qPCR testing confirm compatibility with reverse transcriptases and various DNA polymerases, with no effect on SP6, T7 and T3 RNA polymerases, AMV and M-MLV reverse transcriptases and Taq DNA polymerase. The enzyme stays active from 25 to 60°C across pH 5.0 to 9.0. |
| Intended Use | RNase protection in first-strand cDNA synthesis, polysome isolation, in vitro transcription and cell-free translation |
| Principle / Technology | Non-covalent complex formation with RNase A, B and C that inactivates the enzymes |
| Sample Type | RNA containing reaction systems prone to RNase contamination |
| Specificity | Broad inhibition of RNase A, B and C; active from 25 to 60°C across pH 5.0 to 9.0; low DTT sensitivity |
| Reaction Conditions / Protocol | Add to the reaction system at the working concentration to protect RNA templates and products |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2 KU; 10 KU; 20 KU; 100 KU; 1,000 KU; 20,000 KU |
| Key Features | Higher antioxidant activity than the human inhibitor; compatible with polymerases and reverse transcriptases |
| Purity | Free of exonuclease, nickase and RNase residue |
| Concentration | 40 U/uL |
| Recommended Buffer System | Hepes-KOH, KCl, DTT, non-ionic surfactants and 50% glycerol at pH 7.5 |
| Readouts / Downstream Assays | RT-qPCR performance with protected RNA templates |
For in vitro diagnostic use.
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