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| Product Name | MunI (MfeI) Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0438 |
| Description | Recognising 5 prime C^AATTG 3 prime, this rapid recombinant endonuclease supports molecular cloning, vector construction and plasmid linearisation with digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. The site generates EcoRI compatible cohesive ends, easing adapter strategy design in multi enzyme cloning schemes. Supplied at 20 U/uL, it operates in the unified rapid series buffer at 37°C Dam, Dcm, CpG, EcoKI and EcoBI methylation have no effect, and MfeI is the documented isoschizomer. Note that the enzyme cannot be heat inactivated; use phenol chloroform extraction or column purification to remove it after digestion. |
| Intended Use | Molecular cloning, vector construction and plasmid linearisation with EcoRI compatible cohesive ends |
| Principle / Technology | Site specific cleavage at 5 prime C^AATTG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomer MfeI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | EcoRI compatible overhangs for flexible cloning design; broad methylation tolerance |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion profile on agarose gel |
For in vitro diagnostic use.
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