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| Product Name | MuA Transposase (0.22 ug/uL) |
| Catalog No. | IVDRM-0521 |
| Description | Encoded by the MuA gene of bacteriophage Mu, this transposase catalyses insertion of phage Mu DNA into a host genome as well as excision and relocation to other positions. Working as a tetramer, MuA binds both ends of the transposon to form a stable transpososome, cuts the donor DNA inside this complex and inserts it into the target DNA through a cut and paste mechanism that integrates the chosen DNA fragment into the genome of interest. Compared with the widely used Tn5 transposase, MuA accepts large DNA fragments and shows high randomness of insertion, which makes it well suited to preparing mutant clones and to cloning circular DNA. |
| Intended Use | Preparation of mutant clones and cloning of circular DNA molecules |
| Principle / Technology | Tetrameric transpososome assembly with cut and paste insertion of donor DNA into the target genome |
| Sample Type | Donor DNA fragments and circular target DNA |
| Reaction Conditions / Protocol | Incubate with transposon-ended donor DNA and the target DNA to drive the transposition reaction |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 2.2 ug; 4.4 ug |
| Key Features | Compatible with large DNA fragments; highly random insertion pattern compared with Tn5 |
| Purity | Greater than or equal to 95% (SDS-PAGE); free of exonuclease and nickase residue |
| Concentration | 0.22 ug/uL |
| Source / Origin | Bacteriophage Mu MuA gene |
| Readouts / Downstream Assays | SDS-PAGE purity and gel analysis of transposition products |
For in vitro diagnostic use.
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