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| Product Name | MspI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0437 |
| Description | Cutting the same 5 prime C^CGG 3 prime site as HpaII but insensitive to CpG methylation, this rapid recombinant endonuclease is the methylation tolerant half of the classic HpaII-MspI analysis pair, since Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave its activity unchanged. It supports gene cloning, recombinant vector construction and plasmid linearisation with digestion completed in 5 to 15 minutes. Supplied at 10 U/uL, it runs in the shared rapid series buffer at 37°C Note that the enzyme cannot be heat inactivated; remove it after digestion by phenol chloroform extraction or column purification. The isoschizomers HpaII, HapII and BsiSI share the specificity. |
| Intended Use | Gene cloning, vector construction and plasmid linearisation with methylation tolerant CCGG digestion |
| Principle / Technology | Site specific cleavage at 5 prime C^CGG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomers HpaII, HapII and BsiSI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 T |
| Key Features | Methylation insensitive counterpart to HpaII for paired epigenetic restriction analysis |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Methylation comparative restriction pattern on agarose gel |
For in vitro diagnostic use.
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