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| Product Name | MscI Rapid Restriction Endonuclease |
| Catalog No. | IVDRM-0441 |
| Description | Recognising 5 prime TGG^CCA 3 prime, this rapid recombinant endonuclease produces blunt ended fragments for DNA fragment preparation and completes digestion of plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It runs in the single shared buffer of the rapid series at 37°C and is heat inactivated at 80°C for 20 minutes. Dcm methylation blocks cleavage at overlapping sequences, and the isoschizomers include BalI, MlsI, Mox20I and Msp20I. |
| Intended Use | Rapid blunt ended DNA fragment preparation from plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific blunt cleavage at 5 prime TGG^CCA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dcm methylation blocks cleavage; isoschizomers include BalI, MlsI, Mox20I and Msp20I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | Blunt end output; one shared buffer across the rapid series |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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