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| Product Name | Mouse CD3/CD28 T Cell Activation Magnetic Beads |
| Catalog No. | RESK-0463 |
| Description | T-cell activation depends on a two-signal system. The first signal (antigen recognition signal) is provided when the TCR/CD3 complex on the T-cell surface recognizes the MHC-antigen peptide complex on antigen-presenting cells (APCs); the second signal (co-stimulatory signal) is provided by binding of the CD28 molecule on the T-cell surface to B7 family molecules (e.g., CD80, CD86) on APCs. In vitro, the first signal can be mimicked by an anti-CD3 antibody, which binds the CD3 molecule and activates the TCR signaling pathway, and the second signal can be mimicked by an anti-CD28 antibody, which provides the co-stimulatory signal. Mouse CD3/CD28 T Cell Activation Magnetic Beads are prepared by covalently coupling high-quality anti-mouse CD3 and CD28 antibodies to inert superparamagnetic beads with a particle size of 5 um, mimicking the function of antigen-presenting cells. T cells can be activated and expanded simply and rapidly without feeder cells (antigen-presenting cells) or antigens. Recombinant mouse IL-2 can be used during cell culture to stimulate T-cell expansion. After cell activation or expansion, the beads can be removed with a magnetic stand. |
| Intended Use | For in vitro activation and expansion of mouse T cells, applicable to mouse splenocytes or T-cell subsets, including CD3+ T cells, CD4+ T cells, and CD8+ T cells. |
| Principle / Technology | Anti-mouse CD3 and anti-CD28 antibodies covalently coupled to 5 um inert superparamagnetic beads mimic antigen-presenting cells by simultaneously delivering the TCR/CD3 activation signal and the CD28 co-stimulatory signal, thereby activating and expanding T cells ex vivo. |
| Sample Type | Mouse splenocytes or T-cell subsets, including CD3+ T cells, CD4+ T cells, or CD8+ T cells. |
| Performance Range / Specifications | Simple operation: beads can be added to cells directly after washing and do not need to be removed during activation and expansion. High activation efficiency: 48 hours after bead activation, CD25+CD69+ T cells account for 86.69%. Stable expansion: activated T cells can be cultured in vitro for up to 21 days, with an expansion fold >1000-fold. Activation capacity: 200 uL size for up to 2×10^7 cells; 1 mL size for up to 1×10^8 cells. |
| Reaction Conditions / Protocol | Wash the beads and add them directly to the cells; the beads do not need to be removed during activation and expansion. Recombinant mouse IL-2 can be used during cell culture to stimulate T-cell expansion. After cell activation or expansion, the beads can be removed with a magnetic stand. |
| Components / Formulation | Anti-mouse CD3 and anti-CD28 antibodies covalently coupled to 5 um inert superparamagnetic beads, supplied in PBS (pH 7.4) with 0.1% BSA and 0.1% ProClin-300. |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 200 uL (up to 2×10^7 cells) / 1 mL (up to 1×10^8 cells). |
| Product Form | Liquid suspension of superparamagnetic beads. |
| Concentration | 1 x 10^8 beads/mL. |
| pH Range / Optimal pH | Storage buffer pH 7.4. |
| Expiration Date / Stability | Valid for 1 year when stored at 2-8 °C; do not freeze. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with magnetic stands for bead removal after activation or expansion. |
| Recommended Buffer System | Supplied in PBS (pH 7.4), 0.1% BSA, 0.1% ProClin-300. |
| Application Notes / Precautions | Do not freeze. Beads should be washed before being added to the cells. Recombinant mouse IL-2 is recommended during culture to stimulate T-cell expansion. |
For research use only, not for clinical use.
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