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| Product Name | MnlI Rapid Restriction Endonuclease (5 U/uL) |
| Catalog No. | IVDRM-0436 |
| Description | Recognising the asymmetric site 5 prime CCTC(N)7^ 3 prime, this rapid recombinant endonuclease cleaves seven and six bases away from its recognition sequence on the two strands, a distal cutting pattern useful in gene engineering fragment manipulation. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 5 U/uL, and runs in the shared rapid series buffer with heat inactivation at 80°C for 20 minutes. EcoBI methylation possibly overlapping the site may block cutting, and no alternative named isoschizomers are documented. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Rapid DNA cutting for gene engineering fragment manipulation with distal cleavage |
| Principle / Technology | Asymmetric cleavage 7 and 6 bases outside 5 prime CCTC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation may block cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Distal cleavage pattern; one shared buffer across the rapid series |
| Concentration | 5 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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