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| Product Name | MluI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0435 |
| Description | This rapid recombinant endonuclease cleaves 5 prime A^CGCGT 3 prime, leaving a 5 prime overhang, and digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it performs in the common rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and no alternative named isoschizomers are documented. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime A^CGCGT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Single shared buffer across the rapid series; activity redundancy for difficult templates |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Gel electrophoresis of digested fragments |
For in vitro diagnostic use.
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