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| Product Name | Micrococcal Nuclease (2000 U/uL) |
| Catalog No. | IVDRM-0487 |
| Description | Also known as Micrococcal Endonuclease, S7 Nuclease or MNase, this endonuclease from Staphylococcus aureus degrades single stranded, double stranded, linear and circular DNA or RNA at pH 7 to 10 in the presence of calcium ions, releasing mononucleotides and oligonucleotides with 3 prime phosphate ends. Its ability to digest chromatin into defined fragments makes it valuable for chromatin fragmentation in chromatin immunoprecipitation. Supplied at 2000 U/uL, the preparation is free of protease residue and carries host genomic DNA at less than 1 copy per 2000 U. |
| Intended Use | Chromatin fragmentation for chromatin immunoprecipitation and DNA substrate digestion |
| Principle / Technology | Calcium dependent endonucleolytic digestion of single and double stranded DNA or RNA, releasing 3 prime phosphate ended products |
| Sample Type | Chromatin; single and double stranded DNA or RNA |
| Reaction Conditions / Protocol | Incubate chromatin or nucleic acid substrate with the enzyme in the presence of calcium ions at pH 7 to 10, then stop the reaction |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1600 KU; 320 KU |
| Key Features | Digests many nucleic acid forms; low host genomic DNA residue; suited to chromatin fragmentation |
| Purity | Free of protease residue; host genomic DNA below 1 copy per 2000 U |
| Concentration | 2000 U/uL |
| Source / Origin | Staphylococcus aureus |
| Readouts / Downstream Assays | Fragment size distribution of digested chromatin |
For in vitro diagnostic use.
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