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| Product Name | Methyl Cellulose Solution, 2×, 1.8% |
| Catalog No. | RERE-0384 |
| Description | Methyl cellulose, sometimes transliterated as methocel, is a semisynthetic viscoelastic polymer that turns liquid culture into a gentle semisolid matrix, anchoring colonies of suspension cells in place so they grow as discrete, countable and pickable clones. This autoclaved 2× stock sits at 1.8 percent with a viscosity of 450 cps or higher, and is diluted to produce the working gel for cloning of cells grown in suspension, colony formation assays and colony isolation. |
| Intended Use | Clonogenic culture of suspension cells, colony enumeration and physical retrieval of individual colonies. |
| Principle / Technology | The viscous polymer network immobilises single cells so each founds a spatially separated colony. |
| Specificity | 2× concentrate; 1.8% methyl cellulose; viscosity 450 cps or above; aqueous, autoclaved. |
| Reaction Conditions / Protocol | Dilute with the appropriate complete medium to the working concentration before seeding cells for colony culture. |
| Storage Conditions | 2-8°C; 12 months. |
| Package Specifications | 50 mL, 250 mL |
| Key Features | High-viscosity 2× stock; autoclaved; classic matrix for colony assays and clonal selection. |
For research use only, not for clinical use.
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