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| Product Name | Mesenchymal Stem Cell Serum-Free Culture Medium |
| Catalog No. | RESK-0486 |
| Description | Mesenchymal Stem Cell Serum-Free Culture Medium is a standardized, xeno-free, serum-free medium specifically designed for human mesenchymal stem cells, including umbilical cord, bone marrow, adipose and cord blood derived mesenchymal stem cells. Compared with conventional media supplemented with animal serum, this serum-free medium maintains undifferentiated growth of mesenchymal stem cells, preserves cellular morphology and normal chromosomal karyotype, and ensures a high proliferation rate and differentiation potential of mesenchymal stem cells. |
| Intended Use | Primary and passaged culture of human mesenchymal stem cells (including umbilical cord, bone marrow, adipose and cord blood derived MSCs). |
| Sample Type | Human mesenchymal stem cells (umbilical cord, bone marrow, adipose and cord blood derived), primary and passaged |
| Performance Range / Specifications | 1. Both the serum-free medium and the supplement are free of xenogeneic animal-derived components. 2. Suitable for primary and passaged culture of human mesenchymal stem cells. 3. Supports high expansion capacity and high cell viability. |
| Reaction Conditions / Protocol | 1. Thaw the MSC serum-free supplement at room temperature or 2-8 °C, mix well and let stand for 5 min until fully dissolved before use or aliquoting. Avoid more than 2 freeze-thaw cycles; aliquots may be stored at -20 °C. 2. Add the supplement to the MSC serum-free basal medium to prepare the complete medium. Store the prepared complete medium at 2-8 °C protected from light and use within 2 weeks. 3. Alternatively, thaw the supplement in a 37 °C water bath, then use directly or aliquot and store at -20 °C, avoiding repeated freeze-thaw cycles. 4. A small amount of flocculent material or turbidity may appear after thawing; this is normal and does not affect cell culture performance. Centrifugation (3400 g, 3-5 min) is recommended to remove the flocculent material. |
| Components / Formulation | Two-part system: umbilical cord MSC serum-free basal medium and umbilical cord MSC serum-free culture supplement; both free of xenogeneic animal-derived components. |
| Storage Conditions | Basal medium: store at 2-8 °C, protected from light. Supplement: store at -20 °C, protected from light; long-term storage at -20 °C to -40 °C is acceptable until the expiration date. |
| Shelf Life | 1 year (both basal medium and supplement). |
| Package Specifications | 500 mL |
| Product Form | Liquid basal medium with frozen liquid supplement |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | 1. Basal medium: 1 year at 2-8 °C protected from light. 2. Supplement: 1 year at -20 °C protected from light; may be stored long-term at -20 °C to -40 °C until the expiration date; once thawed, keep at 4 °C for up to 7 days. 3. Prepared complete medium is stable for 14 days at 4 °C. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | 1. Avoid repeated freeze-thaw cycles of the supplement (no more than 2 times). 2. Flocculent material or turbidity after supplement thawing is normal and does not affect performance; centrifuge at 3400 g for 3-5 min to remove it if desired. 3. Use the prepared complete medium within 2 weeks when stored at 2-8 °C protected from light. |
For research use only, not for clinical use.
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