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| Product Name | MEM (Minimum Essential Medium), with Earle's Salts and L-Glutamine |
| Catalog No. | ATR-CCR-0004 |
| Description | The classic Minimum Essential Medium containing Earle's balanced salts for CO2-buffered incubation, providing the minimal nutrient set required for the growth of adherent mammalian cells in monolayer culture. |
| Intended Use | General purpose culture of adherent mammalian cell lines including HeLa, MRC-5, Vero, Wi-38, and other fibroblast and epithelial lines used in virology, vaccine production, and cell biology research. |
| Principle / Technology | Earle's salts provide bicarbonate buffering for 5% CO2 atmosphere; minimal amino acid and vitamin formulation sufficient for basic cell growth; higher calcium concentration supports cell attachment. |
| Detection Method | Cell morphology and confluence by phase-contrast microscopy, growth rate assessment |
| Sample Type | HeLa, MRC-5, Vero, BHK-21, CHO, and other adherent fibroblast and epithelial lines |
| Performance Range / Specifications | Supports steady growth for standard cell lines at 10% FBS supplementation |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement with 10% FBS, 1% penicillin-streptomycin, and 1x MEM Non-Essential Amino Acids for most cell lines; equilibrate in 5% CO2 before use. |
| Components / Formulation | Earle's balanced salts, essential amino acids (13), 8 water-soluble vitamins, 1 g/L glucose, 2.2 g/L sodium bicarbonate, 2 mM L-glutamine, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L, 6 × 500 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin, pH, osmolality, growth promotion with reference cell lines |
| Key Features | Classic minimal formulation; Earle's salts for CO2 incubation; with L-glutamine; standard for virology and vaccine production; reliable and consistent |
For research use only, not for clinical use.
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