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| Product Name | McCoy's 5A Medium, Modified, with L-Glutamine |
| Catalog No. | ATR-CCR-0008 |
| Description | A modified McCoy's 5A medium containing increased glucose and vitamin concentrations with HEPES buffer, formulated for the primary culture of human biopsy tissue and the propagation of fastidious cell lines. |
| Intended Use | Primary cell culture establishment from tissue biopsies; culture of human and animal cell lines including HeLa, primary amniotic cells, and lens epithelial cells; suitable for chromosome analysis after colcemid treatment. |
| Principle / Technology | Elevated glucose, amino acid, and vitamin levels support metabolically active primary cells during establishment; HEPES provides pH stability during lengthy biopsy transport and processing procedures. |
| Detection Method | Primary outgrowth from tissue explants, karyotype quality assessment for cytogenetic analysis |
| Sample Type | Tissue biopsies, primary amniocytes, chorionic villus samples, lens epithelial cells, bone marrow cultures |
| Performance Range / Specifications | Primary explant outgrowth achieved within 7–14 days for most tissue types; supports chromosomal analysis with good metaphase spread |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement with 15–20% FBS for primary cultures; reduce to 10% for established cell lines; equilibrate in 5% CO2. |
| Components / Formulation | McCoy's 5A base with 3 g/L glucose, HEPES buffer, sodium bicarbonate, peptone, bacto-tryptose phosphate, 2 mM L-glutamine, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin, pH, osmolality, primary cell growth validation, chromosomal quality assessment |
| Key Features | Modified for primary cell culture; HEPES buffered; with L-glutamine; supports tissue biopsy growth; validated for cytogenetic applications |
For research use only, not for clinical use.
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