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| Product Name | MC38 Human CCR5 Stable Cell Line |
| Catalog No. | ATR-CCR-0072 |
| Description | A cryopreserved, adherent MC38 cell line stably expressing tag-free human CCR5. The cells are maintained under puromycin selection and are intended for cell-based research assays. |
| Intended Use | For FACS binding assays and cell-based ELISA. |
| Principle / Technology | Stable transfection followed by antibiotic selection; CCR5 expression is qualified using flow cytometry; cell-based ELISA. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 × 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human CCR5 |
| Reaction Conditions / Protocol | Culture in RPMI 1640 with 10% FBS and 3 μg/mL puromycin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% RPMI 1640 and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80°C. |
| Package Specifications | 1 vial; approximately 5 × 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | CCR5 expression verified by flow cytometry against the parental MC38 cell line. |
| Key Features | Stable human CCR5 expression; adherent growth; puromycin resistance; tag-free; suitable for FACS binding assays and cell-based ELISA. |
| Source / Origin | MC38 cells expressing human CCR5 (UniProt P51681). |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays and cell-based ELISA |
| Recommended Buffer System | RPMI 1640 with 10% FBS and 3 μg/mL puromycin |
| Application Notes / Precautions | Maintain puromycin selection during routine culture. Keep the product frozen until cell recovery. |
| Genotype | Stable human CCR5 expression; puromycin-resistant. |
| Alternative Names / Synonyms | CCR5; C-C chemokine receptor type 5 |
| Host Species | Human |
| Tag Information | Tag-Free |
For research use only, not for clinical use.
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