- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | MboI Rapid Restriction Endonuclease (5 U/uL) |
| Catalog No. | IVDRM-0440 |
| Description | Targeting 5 prime ^GATC 3 prime, this rapid recombinant endonuclease is blocked by Dam methylation fully overlapping the site and may also be hindered by EcoBI methylation, so it selectively digests unmethylated GATC sequences, complementing DpnI which cuts only the methylated form. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 5 U/uL, and runs in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation influences cutting while Dcm and EcoKI are tolerated, and the isoschizomers include Bsp143I, Kzo9I, BssMI, DpnII, BstMBI, NdeII and Sau3AI. |
| Intended Use | DNA fragment preparation with selective digestion of unmethylated GATC sites |
| Principle / Technology | Site specific cleavage at 5 prime ^GATC 3 prime blocked by Dam methylation, within 5 to 15 minutes at 37°C |
| Specificity | Dam methylation blocks cleavage; EcoBI methylation blocks cleavage; CpG affects cutting; isoschizomers include Bsp143I, DpnII, Sau3AI and Kzo9I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Methylation selectivity complementary to DpnI; unified buffer across the rapid series |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Gel based analysis of digestion products |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
