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| Product Name | Lyticase for Yeast Cell Wall Lysis (10 U/uL) |
| Catalog No. | IVDRM-0546 |
| Description | Lyticase, short for fungal cell lysing enzyme, is widely used wherever yeast cell walls must be hydrolysed, including genome research, yeast artificial chromosome extraction, yeast transformation, protoplast preparation, yeast cell disruption and industrial yeast inactivation. This preparation is a solution with an activity of 10 U per microlitre and a common working concentration of 0.15 U per microlitre, and the optimum working concentration is tuned to the application, yeast species and cell quantity. For yeast genomic DNA or plasmid DNA extraction the kit-level concentration is used directly. |
| Intended Use | Yeast cell wall hydrolysis for genome research, YAC extraction, transformation, protoplast preparation and cell disruption |
| Principle / Technology | Enzymatic hydrolysis of the yeast cell wall releasing protoplasts and intracellular contents |
| Sample Type | Yeast cell suspensions |
| Specificity | Common working concentration 0.15 U per microlitre, tuned to yeast type and cell quantity |
| Reaction Conditions / Protocol | Suspend cells and add the enzyme at the working concentration appropriate to the application |
| Storage Conditions | -20°C |
| Package Specifications | 1500 U; 3000 U; 10000 U |
| Key Features | Solution format for direct dosing; supports extraction through inactivation workflows |
| Concentration | 10 U/uL |
| Shipping Conditions | Ice pack shipping |
| Readouts / Downstream Assays | Cell wall breakage and protoplast formation readout |
For in vitro diagnostic use.
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