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Lymphocyte Serum-Free Culture Medium

Cat.No: RESK-0484 Datasheet

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Product Name Lymphocyte Serum-Free Culture Medium
Catalog No. RESK-0484
Description Lymphocyte Serum-Free Culture Medium is a basal medium designed for the growth and expansion of blood and immune cells. It is a xeno-free, chemically defined medium with no added growth factors or antibiotics. Suitable cell types include peripheral blood mononuclear cells (PBMCs), peripheral blood lymphocytes (PBL), T cells, NK cells, CIK cells, iNKT cells, hematopoietic stem cells (CD34+), tumor-infiltrating lymphocytes (TIL), macrophages, murine lymphoma cells, bone marrow cells and leukemia cells.
Intended Use Growth and expansion of blood and immune cells, including PBMCs, PBL, T cells, NK cells, CIK cells, iNKT cells, CD34+ hematopoietic stem cells, TIL, macrophages, murine lymphoma cells, bone marrow cells and leukemia cells.
Sample Type Blood and immune cells (PBMCs, PBL, T cells, NK cells, CIK cells, iNKT cells, CD34+ hematopoietic stem cells, TIL, macrophages, murine lymphoma cells, bone marrow cells, leukemia cells)
Performance Range / Specifications 1. Contains phenol red, HEPES, glutamine and sodium bicarbonate; free of animal-derived components.
2. Supports high-density lymphocyte culture without the addition of serum, autologous plasma or serum replacement.
3. In static culture vessels, supports lymphocyte culture at cell densities of 5 x 10^5 to 5 x 10^6 cells/mL.
Cell counting and viability assays performed on days 3, 5, 7 and 9 of culture demonstrate high expansion capacity and high cell viability.
Reaction Conditions / Protocol 1. Use this product without adding serum, autologous plasma or serum replacement; it supports high-density lymphocyte culture.
2. In static culture vessels, it supports lymphocyte culture at cell densities of 5 x 10^5 to 5 x 10^6 cells/mL.
3. Depending on the specific cell type and application, appropriate cytokines, autologous plasma, human AB serum or FBS may be added to significantly enhance cell proliferation.
Components / Formulation Chemically defined, xeno-free basal medium containing phenol red, HEPES, glutamine and sodium bicarbonate; no added growth factors or antibiotics.
Storage Conditions Store at 2-8 °C. Protect from light.
Shelf Life 12 months.
Package Specifications 50 mL
1000 mL
Product Form Liquid medium
Expiration Date / Stability Stable for 12 months when stored at 2-8 °C protected from light.
Regulatory / Compliance For research use only.
Application Notes / Precautions 1. No serum, autologous plasma or serum replacement is required for use.
2. Appropriate cytokines, autologous plasma, human AB serum, FBS or other supplements may be selected according to the specific cell type and application requirements to significantly improve cell proliferation.

For research use only, not for clinical use.

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