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| Product Name | LwaCas13a Nuclease for RNA Targeting (10 uM) |
| Catalog No. | IVDRM-0589 |
| Description | An RNA-guided RNA endonuclease from Leptotrichia wadei, LwaCas13a depends on RNA-mediated recognition, so that guided by a crRNA and given a suitable protospacer flanking site it specifically recognises and cuts target single-stranded RNA. The enzyme also carries a target ssRNA-dependent trans, or collateral, cleavage activity that is triggered only once LwaCas13a, crRNA and target ssRNA form a ternary complex. Once activated it not only cuts the target RNA efficiently but also degrades any single-stranded RNA in the reaction, and this signal amplification behaviour underpins rapid nucleic acid detection kits in molecular diagnostics. The untagged 138.5 kDa protein is supplied at 10 uM with at least 95% purity in a Tris-HCl, NaCl, DTT, EDTA and glycerol buffer at pH 8. |
| Intended Use | Nucleic acid detection kit development that exploits trans cleavage signal amplification in molecular diagnostics |
| Principle / Technology | RNA-guided RNA endonuclease activity with collateral trans cleavage after ternary complex formation |
| Sample Type | Target single-stranded RNA carrying an appropriate protospacer flanking site |
| Specificity | Target ssRNA-dependent trans cleavage that is activated only after ternary complex formation |
| Reaction Conditions / Protocol | Combine with crRNA and the target ssRNA, then trigger collateral cleavage for signal readout |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 700 pmol; 3500 pmol |
| Key Features | Target-dependent trans cleavage for amplified detection signal; untagged 138.5 kDa protein |
| Purity | Greater than or equal to 95% |
| Concentration | 10 uM |
| Molecular Weight | 138.5 kDa |
| Source / Origin | Leptotrichia wadei |
| Recommended Buffer System | Tris-HCl, NaCl, DTT, EDTA and glycerol at pH 8 |
| Readouts / Downstream Assays | Fluorescent reporter signal from collateral single-stranded RNA cleavage |
For in vitro diagnostic use.
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