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| Product Name | Leibovitz's L-15 Medium |
| Catalog No. | RESK-0639 |
| Description | Leibovitz's L-15 Medium (L-15) employs a buffering system composed of salts, basic (free) amino acids and galactose. This system reduces the accumulation of acidic by-products and helps maintain a stable culture pH, making the medium well suited for cell culture in non-CO2-equilibrated environments, i.e., without a CO2 incubator. It is commonly used for the in vitro culture of HEp-2 and LLC-MK2 cells, primary embryonic tissues, adult tissues and a variety of viruses. This product is sterilized by 0.1 um filtration and can be used directly for cell culture. Contains: galactose, L-glutamine, sodium pyruvate and phenol red. Without: glucose, sodium bicarbonate and HEPES. |
| Intended Use | For cell culture in non-CO2-equilibrated environments (no CO2 incubator required), including in vitro culture of HEp-2 and LLC-MK2 cells, primary embryonic tissues, adult tissues and various viruses. |
| Principle / Technology | Buffering is achieved with salts, basic free amino acids and galactose instead of the conventional sodium bicarbonate/CO2 system, reducing acidic by-product accumulation and stabilizing culture pH in ambient-atmosphere conditions. |
| Performance Range / Specifications | Formulation with galactose, L-glutamine, sodium pyruvate and phenol red; without glucose, sodium bicarbonate and HEPES. Sterilized by 0.1 um filtration. |
| Components / Formulation | Amino Acids Glycine 200 mg/L L-Alanine 225 mg/L L-Arginine 500 mg/L L-Asparagine 250 mg/L L-Cysteine 120 mg/L L-Glutamine 300 mg/L L-Histidine 250 mg/L L-Isoleucine 250 mg/L L-Leucine 125 mg/L L-Lysine 75 mg/L L-Methionine 75 mg/L L-Phenylalanine 125 mg/L L-Serine 200 mg/L L-Threonine 300 mg/L L-Tryptophan 20 mg/L L-Tyrosine 300 mg/L L-Valine 100 mg/L Vitamins Choline Chloride 1 mg/L Calcium D-pantothenate 1 mg/L Folic Acid 1 mg/L Niacinamide 1 mg/L Pyridoxine HCl 1 mg/L Riboflavin 0.1 mg/L Thiamine HCl 1 mg/L i-Inositol 2 mg/L Inorganic Salts Calcium Chloride 140 mg/L Magnesium Chloride 93.7 mg/L Magnesium Sulfate 97.67 mg/L Potassium Chloride 400 mg/L Potassium Phosphate Monobasic 60 mg/L Sodium Chloride 8000 mg/L Sodium Phosphate Dibasic 190 mg/L Other Components D-Galactose 900 mg/L Phenol Red Na 10 mg/L Sodium Pyruvate 550 mg/L |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year when stored at 2-8 °C, protected from light. |
| Package Specifications | 500 mL |
| Product Form | Liquid, 1x ready-to-use cell culture medium |
| Quality Control | Sterilized by 0.1 um filtration. |
| Regulatory / Compliance | For research use only. |
| Recommended Buffer System | Salt, free basic amino acid and galactose buffering system; no sodium bicarbonate, suitable for use without a CO2 incubator. |
| Application Notes / Precautions | Designed for culture without CO2 equilibration; do not supplement with sodium bicarbonate unless specifically required. Protect from light during storage. |
For research use only, not for clinical use.
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